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. 2009 Oct 27;150(12):5557–5565. doi: 10.1210/en.2009-0442

Figure 2.

Figure 2

Effect of high d-glucose on prorenin and renin expression in RMCs. High-glucose exposure increased prorenin mRNA (A) and prorenin and renin protein (B). Renin protein (C) increased in RMCs culture supernatants in response to high-glucose exposure as demonstrated by immunoprecipitation (IP). Immunofluorescence staining of prorenin and renin (D). NEG, negative control (no antibody control); control, 30 mm l-glucose; glucose, 30 mm d-glucose; KD, knockdown; WB, Western blot; Ab, antibody.