Figure 5.

CNS resveratrol administration improves hepatic PEPCK expression and Pyruvate-induced hyperglycemia. Pepck and G6Pase mRNA level (determined by quantitative real-time PCR) (A) and PEPCK and β-actin (used as loading control) protein levels (determined by Western blot analysis) (B) were assessed in liver of HC-saline, HC-resveratrol, and chow-fed, age-matched, C57BL/6 male mice (n = 6–7 for each group). C, Blood glucose levels over time after a pyruvate tolerance test in a different cohort 5 wk after treatment (n = 7 for each group). Error bars, sem. Statistical analyses were done using one-way ANOVA (Tukey’s post test). *, P < 0.05; **, P < 0.01; ***, P < 0.001 vs. HC-saline mice. D, A proposed model of action through which brain-specific SIRT1 activators may suppress diet-induced brain inflammation leading to improved hyperglycemia and hyperinsulinemia.