Figure 1.

VEGF and PR mRNA expression in cancer cell lines grown in 95% air-5% CO₂. ECC-1, MCF-7, and ZR-75 cells were treated with vehicle (0 h) or 10 or 100 nm E₂ for 2 or 4 h, as indicated. VEGF and PR mRNA expression were measured by real-time RT-PCR, as described in Materials and Methods. A, ECC-1 cells (mean ± sem, n = 6 replicate cultures/group; *, P < 0.01, **, P < 0.0001 vs. all other groups). B, MCF-7 cells (mean ± sem, n = 5 replicate cultures/group; *, P < 0.001 vs. all other groups). C, ZR-75 cells (mean ± sem, n = 5 replicate cultures/group; *, P < 0.05, #, P < 0.05 vs. all other groups).