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. 2009 Oct 9;150(12):5405–5414. doi: 10.1210/en.2009-0884

Figure 3.

Figure 3

Effect of CoCl2 and E2 on ERα expression, subcellular localization, and phosphorylation in ECC-1 cells. A, Cytoplasmic and nuclear localization of total ERα, p (phospho)-ERαSer167, and Akt, determined by Western blot analysis (10 μg protein/lane). B, Quantification of total ERα by densitometry after normalization to Akt (mean ± sem, n = 5 replicate cultures/group; *, P < 0.0001, **, P < 0.001 vs. all other groups). C, Quantification of p-ERα Ser167 by densitometry after normalization to Akt (mean ± se, n = 5 replicate cultures/group; *, P < 0.01 vs. all other groups).