Figure 4.
Effect of CoCl2 and E2 on VEGF, adrenomedullin, and PR mRNA expression. Cells were treated as described in Fig. 2C and VEGF, adrenomedullin, PR, and 18S mRNA levels measured by RT-PCR. A and B, VEGF mRNA expression. A, Conventional RT-PCR representative gels. B, Real-time RT-PCR results (mean ± sem, n = 3 replicate cultures/group; *, P < 0.05, **, P < 0.0001 vs. all other groups). C and D, Adrenomedullin mRNA expression. C, Conventional RT-PCR representative gels. D, Real-time RT-PCR results (mean ± sem, n = 3 replicate cultures/group; *, P < 0.001, **, P < 0.0001 vs. all other groups). E and F, PR mRNA expression. E, Conventional RT-PCR representative gels. F, Real-time RT-PCR results (mean ± sem, n = 3 replicate cultures/group; *, P < 0.01, **, P < 0.0001 vs. all other groups).
