Figure 5.

Effect of CoCl₂ and E₂ on recruitment of HIF-1α and ERα to the VEGF gene promoter. ChIPs were carried out using normal rabbit serum (NRS) or specific antibodies for ERα or HIF-1α (see Materials and Methods). A, Primers for the −1216 to −883 region of the human VEGF promoter, which contains the HRE site to which HIF-1α binds, and the −222 to +139 region of the human VEGF promoter, which contains the Sp1 sites to which ERα binds, were used for PCR. B, Representative conventional PCR gels. C, Real-time PCR results (mean ± sem, n = 6 replicate cultures/group; *, P < 0.05, #, P < 0.01 vs. other treatment groups). IP, Immunoprecipitation.