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. 2009 Oct 9;150(12):5362–5372. doi: 10.1210/en.2009-0509

Figure 4.

Figure 4

Role of aPKC activity in NF-κB pathway activation by LPS in cultured N-43/5 hypothalamic neurons. A and B, N-43/5 cells were cultured overnight in serum-free medium followed by treatment with INH (0, 10, or 30 μm), LPS (1 μg/ml), or the combination for 1 h. Quantification of IκBα (A) and IL-6 (B) gene expression by real-time PCR with relative to GAPDH mRNA and normalizated to INH 0 μm control cells. Bars, Mean + se from three experiments with duplicate wells. *, P < 0.05 compared with all other treatments; **, P < 0.05 compared with control treatments and LPS alone.