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. 2009 Oct 9;150(12):5478–5487. doi: 10.1210/en.2009-0283

Figure 1.

Figure 1

GHRP-2 effect on atherosclerosis and vascular superoxides in ApoE−/− mice. A, En face analysis of atherosclerosis. Representative images of aortas obtained from mice treated twice per day with either PBS or GHRP-2 and stained with Oil Red O (ORO). Atherosclerotic burden is expressed as a percentage of plaque area to total area. n = 15 for each group. B, Atherosclerosis in cross-sections of the aortic valve. Paraffin-embedded cross-sections were stained with H&E and cryosections with Oil Red O. Scale bar, 200 μm. n = 25 (PBS) or n = 28 (GHRP-2). C, Superoxide levels in ascending aorta. Cross-sections of the ascending aorta were stained with DHE to visualize superoxide. For each aorta, a serial section was also treated with PEG-SOD to establish a background level of fluorescence. Images were taken at ×200 magnification. Quantitative data are expressed relative to PBS-treated mice. n = 7 (PBS) or n = 6 (GHRP-2). *, P < 0.01 vs. PBS. D, 12/15-Lipoxygenase immunohistochemistry. Sections were stained with an anti-12/15-lipoxygenase antibody or normal rabbit IgG as a negative control, and immunoreactive area is expressed as a ratio of positive (brown) area to total plaque area determined by the adjacent H&E-stained slide. n = 5 per group. *, P < 0.05 vs. PBS. NS, Not significant.