Figure 4.

Subcellular localization of GLUT9 in MIN6 cells. A and B, GLUT9 protein expression in MIN6 subcellular fractions. MIN6 cells were homogenized and subcellular fractions were isolated: PM, HDM, and LDM. Subcellular fractions were analyzed by Western blot using antibodies to mGLUT9a (A) and mGLUT9b (B). Fractions were also analyzed by Western blot using antibodies to Na/K-ATPase (PM), calnexin (HDM), and GM130 (LDM) to determine the relative purity of the fractions (C). Results are representative of three independent experiments.