Table 3.
Resistance of the microorganisms studied to inhibitors present in feedstock hydrolysates
| Production host | Furfural | HMF | Acetate | NaCl | MgSO4 | Growth at pH4 |
|---|---|---|---|---|---|---|
| E. coli | 1 | 2 | 5 | 40 | 80 | - |
| C. glutamicum | 0.5 | 2 | 10 | 40 | > 80 | - |
| S. cerevisiae Bioscreen* | 1 | 1 | 5 | 20 | > 80 | +/- |
| S. cerevisiae Micro-Oxymax* | n.d. | 1 | n.d. | 20 | n.d. | n.d. |
| P. stipitis | 1 | 2 | 5 | 40 | > 80 | +/- |
| T. reesei | 1 | 5 | 20 | 100 | > 80 | + |
| A. niger | 1 | 5 | 20 | 100 | > 80 | + |
The concentration (g/l) of the inhibitor is shown at which inhibition results in <50% of the growth rate/CO2 production rate without inhibitor.
*In order to check correlation of the methods, S. cerevisiae was evaluated using both the Bioscreen and Micro-Oxymax method (see Methods).
n.d. = not determined
+ good growth
+/- moderate growth
- no or very slow growth