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. Author manuscript; available in PMC: 2009 Dec 17.
Published in final edited form as: Dev Neurobiol. 2009 Aug;69(9):568–582. doi: 10.1002/dneu.20727

Figure 4.

Figure 4

Treatment of differentiating hippocampal neurons with unconjugated bilirubin (UCB) reduces neuronal arborization. Examples of embryonic hippocampal neurons treated with vehicle (A and A′), UCB 50 μM (B and B′), or UCB 100 μM (C and C′) for 24 h at 1 DIV and fixed and stained at 3 DIV (A–C) or 9 DIV (A′–C′). Cells were visualized by EGFP fluorescence (shown in black and white and inverted such that EGFP appears black), and neurites were manually traced using ImageJ v1.40 and the NeuronJ plugin v1.4.0. Graphs quantify the effect of UCB on total neurite output at 3 DIV (D) and 9 DIV (D′). *p < 0.05 and **p < 0.01 vs. vehicle. y axis indicates length in microns.