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. Author manuscript; available in PMC: 2009 Dec 17.
Published in final edited form as: Dev Neurobiol. 2009 Aug;69(9):568–582. doi: 10.1002/dneu.20727

Figure 6.

Figure 6

Treatment of differentiating hippocampal neurons with unconjugated bilirubin (UCB) reduces axonal arborization. Embryonic hippocampal neurons were treated with vehicle, UCB 50 μM or UCB 100 μM for 24 h at 1 DIV and fixed and stained at 3 or 9 DIV. Cells were visualized by EGFP fluorescence and neurites were manually traced using ImageJ v1.40 and the NeuronJ plugin v1.4.0. Graphs show the effects of UCB on (A) axonal length, (B) number of axonal branches, and (C) length of axonal branches at 3 and 9 DIV, from at least three independent experiments. *p < 0.05 and **p < 0.01 vs. respective vehicle.