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. 2009 Dec 3;10:580. doi: 10.1186/1471-2164-10-580

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Copyright ©2009 Tabone et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Real-time PCR showing biphasic amplification in TSP assays. Reactions were performed under TSP cycling conditions using A. 0.1 μM of LS primer pair only (forward 5'-CTACTGGAAGGCCGGCAAGC and reverse 5'-CGCATAAACCTCAACATCTGAGCA), B. 0.5 μM of NLS primer pair only (forward 5'-GCGTTAAGCATACAGTTTTAGTA and reverse 5'-GGGCCTGAAACCAACC). Underlined sequence indicates the non-complementary 5'-tail region. C. 0.1 μM of LS primer pair and 0.5 μM of NLS primer pair, and D. negative control reaction with 0.1 μM of LS primer pair and 0.5 μM of NLS primer pair without genomic DNA template.