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The effect of constant annealing temperature thermal cycling on TSP genotyping efficiency. Reactions were performed with an annealing temperature of 58°C for 35 cycles using 0.1 μM of LS primers (forward 5'-CGAGGATTGGCTCAAGACGC and reverse 5'-GCAGCGTTCTTAGGACTGGCA) and 0.5 μM of NLS primer (forward 5'-GGCCAGAGTAAGTTGCTGAA and reverse 5'-CGGTTGATCCCGTAGGTG). Underlined sequence indicates the non-complementary 5'-tail region. M represents a pUC19/HpaII DNA size ladder.
