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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Nat Immunol. 2009 Oct 22;11(1):76–83. doi: 10.1038/ni.1825

Figure 4.

Figure 4

FISH of adherent bacteria in mouse distal small intestine. a. Sections (3 micron) of distal small intestine from vendor-obtained wild-type (WT) mice or offspring from DEFA5 tg (+/−) breeding pairs were hybridized with a mixture of oligonucleotide probes recognizing SFB (6Fam-SFB) and total bacteria (TR-Bact338)36, and visualized by fluorescence microscopy23. Where indicated, mice were analyzed 14 days post-gavage with feces containing SFB. All mice were on a pure FVB background. b. Sections of distal small intestine (3 microns) from offspring from Mmp7+/− breeding pairs were hybridized and visualized as in (a). All mice in (b) were on a pure B6 background. Arrows point to SFB bacteria. Arrowheads point to non-SFB bacteria. Non-specific autofluorescence staining is noted in the mucus when viewing with FITC filters (SFB panels), and does not correlate with any specific bacteria (visualized using Texas Red filters-All Bacteria panels).