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Stable over expresses GASC1 in MCF10A cells with the pLenti6/V5-GASC1 construct (MCF10A-GASC1). Over expression of GASC1 mRNA and protein in this cell line was confirmed with (A) semiquantitative RT-PCR and (B) western blot assays. (C) In vitro growth rate of the MCF10A cells that stably over express GASC1 relative to MCF10A control cells in insulin deficient media. Cells were seeded into 35-mm culture wells and grown in the absence of insulin-like growth factors.
