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. 2010 Jan 8;6(1):e1000807. doi: 10.1371/journal.pgen.1000807

Figure 3. Functional rescue of mutant p53 and MTHFR in yeast.

Figure 3

(A) Yeast p53 tester strain yIG397 (HSP26), or LS2 (hsp26Δ) were transformed with an expression plasmid expressing the indicated p53 alleles. Stationary phase cultures of yeast expressing the wild type and indicated mutant were diluted 1∶1000 in SC-ura -ade media grown in 15 ml tubes with aeration at 30°C for 24 hours. Where indicated, 4% ethanol or 50 µM bortezomib were added to the media. (B) Yeast strain yIG397 carrying the indicated p53 alleles was grown in adenine containing media with or without 4% ethanol. Lysates were then probed by Western Blot with the indicated anti-bodies. (C) Yeast strains yIG397 and LS2 expressing the indicated p53 alleles were grown in adenine containing media supplemented with 4% ethanol when indicated. Lysates were prepared and immunoprecipitation was performed with anti-p53 antibody and Hsp26 present in the immune complexes was analyzed by immunoblot. (D) Yeast MTHFR tester strain XSY3-1A (met11Δ) were transformed with expression plasmids expressing the indicated MTHFR allele.