Table 1. Relative levels are shown for CBS protein and activity in yeast.

Protein Levels					Activity Levels
Mutants	No treatment	Ethanol	Bortezomib	ΔHsp26	No treatment	Ethanol	Bortezomib	ΔHsp26
WT	1.0	2.2	1.6	1.2	100	149	120	96
G307S	0.9	0.4	2.4	1.1	1	3	2	57
T262M	1.1	1.2	2.2	1.2	3	44	68	3
D376N	1.4	0.6	2.2	1.4	1	3	2	50
T353M	0.7	1.1	2.3	1.6	3	25	44	37
A231L	0.3	1.4	2.1	1.2	1	45	3	35
T191M	0.7	0.3	6.1	1.7	1	4	2	48
G151R	0.7	0.2	1.6	0.8	1	4	35	1
L101P	0	0	1.2	0.9	1	4	28	1
N228S	1.4	0.7	2.1	1.6	2	4	29	40
Q528K	1.5	0.7	2.8	2.3	1	3	17	1
L496P	0	0	1.9	0.9	1	3	2	66
G116R	0	0	1.2	0	1	3	3	1
A114V	0	0	ND	ND	14	15	70	13
V320A	1.5	0.8	1.8	1.8	2	2	84	54
R224H	0.3	0.3	2.2	1.2	1	55	28	1
V168M	0.3	0.3	1.9	1.0	1	4	2	67
A226T	1.0	ND	1.6	1.6	2	2	22	68
I278T	0.2	0.6	1.4	0.6	2	65	49	59

Protein is normalized to 1 while enzyme activity is normalized to 100 for wild-type human CBS.