Skip to main content
. 2009 Dec 31;4(12):e8443. doi: 10.1371/journal.pone.0008443

Figure 5. Demonstration of functional vessels in vivo using Matrigel plug and dorsal window chamber.

Figure 5

(A) Matrigel plug with hESC-ECs were implanted subcutaneous injection in the dorsal region of 8-week-old SCID mice. (I) HE stain of Matrigel plug. Some of these microvessels have mouse blood cells in their lumen. (II–IV) 14-day Matrigel plugs were stained with anti-human CD31 (red) and anti-GFP (green) antibodies, showing microvessels that are immunoreactive with these human-specific antibodies. Scale bars = 20 µm. (B) Dorsal window chamber model in SCID mice. GFP+ hESC-ECs were cultured in a mix of collagen and Matrigel for 1 day (left, upper panel), and implanted into dorsal windows in SCID mice (middle, upper panel). Images were taken at day 2, 14 and 21 after implantation. After 14 days, Angiosense 680 was injected by tail vein to highlight perfused vessels within the dorsal window. Green, hESC-ECs expressing GFP; red, functional blood vessels with contrast enhanced by Angiosense 680. Scale bars = 20 mm (middle, upper panel), 20 µm (others).