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. Author manuscript; available in PMC: 2010 Jul 21.
Published in final edited form as: Biochemistry. 2009 Jul 21;48(28):6664–6673. doi: 10.1021/bi8022653

Figure 1.

Figure 1

(a) Assembly kinetics measured by FRET following addition of GTP to 10 μM FtsZ in 100 mM KAc, 350 mM KAc, or 500 mM KAc buffer (pH 7.7). Assembly is measured by the decrease in donor fluorescence, (b–d) Negative stain EM of fluorescein-labeled FtsZ filaments in buffers containing 5 mM Mg (pH 7.7) and increasing concentrations of potassium. The bar is 100 nm.