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Mixture of 5 μM donor and 5 μM acceptor FtsZ pre-assembled with 100 μM GTP in HMK buffer containing 100,350, or 500 mM KAc buffer. Disassembly was induced by (a) addition of a 20-fold excess of GDP (2 mM) or (b) a 15-fold dilution. Disassembly is measured by the increase in donor fluorescence. The solid lines show the fits for a single-exponential decay. The fluorescence scales were reset between panels a and b and should not be compared.
