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. Author manuscript; available in PMC: 2010 Jul 21.
Published in final edited form as: Biochemistry. 2009 Jul 21;48(28):6664–6673. doi: 10.1021/bi8022653

Figure 5.

Figure 5

Effects of increasing Mg concentration in HMK100. (a) Filament subunit exchange at steady state. Curves in panel a are the best fit with a single or double exponential. (b) The fit to exchange in 10 mM Mg was improved somewhat using a double-exponential decay. (c and d) Disassembly kinetics at variable Mg concentrations, induced by excess GDP (c) or dilution (d). (e and f) EM shows bundling of filaments in 10 and 20 mM Mg. The bar is 100 nm. See Figure 1b for EM in 5 mM Mg, which generates primarily one-stranded protofilaments.