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. Author manuscript; available in PMC: 2009 Dec 18.
Published in final edited form as: Biochemistry. 2009 Sep 22;48(37):8869–8878. doi: 10.1021/bi900968a

Figure 6.

Figure 6

Effects of diamide on IDH in vivo. Yeast idh1Δidh2Δ transformants expressing the wild-type enzyme or the indicated mutant forms of IDH were grown in YP galactose medium to culture A600 nm values of ~2. Cells were harvested immediately after (times 0) and at the times indicated following the addition of 2 mM diamide to the cultures. Cells from control cultures (ctl lanes) containing no diamide were harvested after 5 h of growth. Cellular protein extracts were obtained by glass bead lysis, and ~5μg samples were electrophoresed under nonreducing conditions. Immunoblot analysis was conducted using an antiserum for the IDH holoenzyme (3). Positions of molecular size standards are indicated on the right of the figure.