Figure 7. AFP;Akt2^lox/lox mice on lard HFD have decreased hepatic triglyceride levels and SREBP-1c expression.

Male mice were started on lard HFD at approximately 6 weeks of age and sacrificed after 1 month on diet under fed conditions along with age-matched chow-fed controls. A. Hepatic triglycerides. B. Hepatic gene expression as measured by rtPCR. Data are presented as mRNA expression relative to that of TBP and normalized to expression in Akt2^lox/lox chow-fed, which is set to 1.0 using the ddC_T method. All values are expressed as mean ± SEM. n=5-6; *p<0.05 vs Akt2^lox/lox chow and **p<0.05 vs Akt2^lox/lox HFD by one-way ANOVA using Newman-Keuls post-test. C. Western blot of phospho-Akt (p473 and p308) and downstream signaling targets in hepatic lysates from Akt2^lox/lox and AFP;Akt2^lox/lox mice. 8-week old male mice were fasted overnight and IP injected with either saline or 1U/kg insulin, then sacrificed after 20 minutes. Each lane represents an individual mouse. Loading controls are included when blots from different gels were used for phospho-protein and total protein, though the same protein extracts were used. The arrow indicates Akt2 as there is a slightly more mobile non-specific band.