Figure 7.

JNK1 is required for estrogen antagonist-induced HIC1 expression and promoter recruitment of prohibitin. A, MCF7 cells were transfected with a JNK1 expression vector, followed by immunoblot analysis using the antibodies indicated. B, MCF7 cells were transfected with JNK1 siRNA or control siRNA, followed by immunoblot (IB) analysis by the indicated antibodies. C, MCF7 cells with JNK1 depleted by siRNA were treated with vehicle control of 4HT for 4 h, followed by ChIP assay using the indicated antibodies and real-time PCR. Amplification of input DNA (total) produced similar levels of product in all lanes.