Increased pituitary E2F1 and PTTG1 expression in Rb+/− mice. A, Representative Western blots of pituitary E2F1 and PTTG1 expression in Rb+/− and paired WT mice (3–15 months old). B, E2F1 and PTTG1 immunoreactivity in Rb+/− and WT pituitary. E2F1 staining is higher in 2-month-old Rb+/− (c) than paired WT (a) mouse pituitary, and hPTTG1 expression is enhanced in 2-month-old Rb+/− (d) vs. paired WT (b) mouse pituitary. Image size, 750 × 750 μm; corner insets size, 100 × 100 μm. Abundant E2F1 (e) and PTTG1 expression (f) in 6-month-old Rb+/− pituitary tumor. Images depict a combined scan field sized approximately 1000 × 1250 μm. Red signal, E2F1 staining; green signal, PTTG1 staining; blue signal, DAPI nuclear staining; yellow signal, autofluorescence background.