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. 2009 Dec 30;4(12):e8488. doi: 10.1371/journal.pone.0008488

Figure 2. Immunoreactivity for phosphorylated extracellular signal-regulated kinases 1 and 2 (P-Erk1/2) in the hypothalamus and co-expression of c-Fos and tyrosine hydroxylase (TH) immunoreactivity in the brainstem after PP injection.

Figure 2

(A) Brightfield micrograph showing P-Erk1/2 immunoreactivity in the arcuate nucleus of the hypothalamus (ARC) at 30 minutes after i.p. injection of PP in wild type mice. Scale bar = 200 µm. (B) Higher magnification of the boxed area from A. Scale bar  = 5 µm. (C, E) Fluorescence micrographs of the nucleus tractus solitarius (NTS) and area postrema (AP) respectively, 30 minutes after i.p. injection of PP. Scale bar  = 40 µm. (D, F) Higher magnifications of the NTS and AP, respectively, from the boxed areas in C and E. Scale bar  = 25 µm. White arrows indicate neurons positive for c-Fos immunoreactivity only (red fluorescence); white arrowheads indicate neurons positive for TH immunoreactivity only (green fluorescence); blue arrows indicate neurons double-labeled for c-Fos and TH. 4V, fourth cerebral ventricle; cc, central canal.