Table 1. Number of c-Fos-like immunoreactive neurons in the brainstem and hypothalamus of wild type and Y4 receptor knockout (Y4 −/−) mice at 30 or 90 minutes after i.p. injection of saline or pancreatic polypeptide (PP).
Regions | Wild type Saline (30 min) | Wild type PP (30 min) | Wild type PP (90 min) | Y4 −/− PP (30 min) |
NTS | 10±2 | 23±6*# | 14±3 | 8±2 |
AP | 3±1 | 26±5*# | 5±2 | 3±1 |
ARC | 9±2 | 38±5*# | 13±3 | 8±1 |
PVN | 15±2 | 42±6*# | 60±16* | 14±3 |
VMHDM | 20±2 | 71±5* | 67±6* | 19±3 |
LHA | 19±4 | 43±8*# | 78±29* | 17±3 |
Data are means ± SEM of 4–6 mice per groups.* P<0.05 versus saline-injected wild type mice; # P<0.05 versus PP-injected wild type mice (90 minutes). NTS: nucleus tractus solitarus; AP: area postrema; ARC: arcuate nucleus of the hypothalamus; PVN: paraventricular nucleus of the hypothalamus; VMHDM: dorsomedial part of the ventromedial nucleus of the hypothalamus; LHA: lateral hypothalamic area.