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. Author manuscript; available in PMC: 2010 Dec 18.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Oct 12;390(3):662–666. doi: 10.1016/j.bbrc.2009.10.024

Figure 3.

Figure 3

Immunoprecipitation of mProVII variants tagged with GFP and/or RFP. mProVII molecules were exposed to the anti-GFP antibodies. Subsequently, the mProVII-anti-GFP complexes were precipitated and analyzed by Western blot assays for the presence of RFP. Symbols: he-G2575R-GFP/WT-RFP, he-G2622Q-GFP/WT-RFP, and he-G2623C-GFP/WT-RFP; heterogeneous mProVII molecules consisting of RFP-tagged WT chains and GFP-tagged mutant chains, ho-G2623C-GFP & ho-WT-RFP; a control sample that includes a mixture of the homogeneous G2623C mProVII mutant tagged with GFP and homogeneous WTmProVII tagged with RFP, +/− indicates the presence or the absence of the anti-GFP antibodies in the analyzed samples. The last lane contains the RFP-tagged homogeneous WTmProVII that serves as a positive marker for immuno-detection of RFP.