Figure 1.

ARMS PCR analysis for APC I1307K. Agarose gel electrophoresis of ARMS PCR products. The upper row (← I) shows I1307-specific (wild-type) PCR products, and the lower row (K →) shows K1307-specific products at 255 bp. In any given numbered lane the products in the two rows are from the same individual; lanes labeled “M” are 100-bp ladder DNA marker. Lanes 1–4, 6, and 7 are from I1307K-negative individuals; lanes 5 and 8 are from I1307K-carrying patients 2–33 and 2–49, respectively; lane 9 is an I1307K-positive control; lanes 10 and 11 are from E1317Q-carrying patients 2–80 and 2–82, respectively; and lane 12 is a known homozygous, wild-type negative control.