Figure 1. Interactions of platinated TFOs with DNA duplex 4RY.

(a) Solutions containing 10 μM platinated TFO and 1 μM duplex 4RY, whose purine strand was labeled with ³²P-phosphate, were incubated at 4°C in triplex buffer that contained 100 mM MOPS, pH 6.5, 100 mM sodium acetate, 2.5 mM magnesium acetate, and were then subjected to electrophoresis on a 20% native polyacrylamide run at 4°C and 100 V. (b) Solutions containing platinated TFO and 1 μM duplex 4RY, whose purine strand was labeled with ³²P-phosphate (shown in red), were incubated at 37°C for 48 hrs in triplex buffer and were then subjected to electrophoresis on a 20% denaturing polyacrylamide gel. Percent adduct formation is shown below the corresponding lane (% X-L). (c) Solutions containing platinated TFO and 1 μM duplex 4RY, whose pyrimidine strand was labeled with ³²P-phosphate (shown in red), were incubated at 37°C for 48 hrs in triplex buffer and were then subjected to electrophoresis on a 20% denaturing polyacrylamide gel. Percent adduct formation is shown below the corresponding lane (% X-L).