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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Bioconjug Chem. 2009 Dec;20(12):2222–2230. doi: 10.1021/bc900008s

Figure 4. Adduct formation between platinated TFOs and DNA duplexes.

Figure 4

(a) Sequences of DNA duplexes. Potential sites of platination are shown in bold. (b) Percent adduct formation between platinated TFOs t1d, t2d or t3d and the purine or pyrimidine strands of the DNA duplexes. Solutions containing 1 μM 32P-labeled duplex and 2 μM platinated TFO were incubated in triplex buffer at 37°C for 48 hrs and then analyzed by PAGE on a 20% denaturing gel. The amount of cross-linking was quantitated by phosphorimaging the gel.