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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Bioconjug Chem. 2009 Dec;20(12):2222–2230. doi: 10.1021/bc900008s

Figure 5. Sequence dependent interactions between platinated TFOs and DNA duplexes.

Figure 5

(a) Sequence of the scrambled duplex, SRY. (b) Solutions containing 1 μM 32P-labeled duplex SRY or 1 μM 32P-labeled duplex 4RY and 2 μM platinated TFO were incubated in triplex buffer at 37°C for 48 hrs and then analyzed by PAGE on a 20% denaturing gel. (c) Solutions containing 1 μM 32P-labeled duplex 4RY, 2 μM platinated TFO and increasing concentrations of competitor non-platinated TFO were incubated in triplex buffer at 37°C for 48 hrs and then analyzed by PAGE on a 20% denaturing gel. The percentage of adduct formed by each platinated TFO versus competitor concentration was determined.