Skip to main content
. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Bioconjug Chem. 2009 Dec;20(12):2222–2230. doi: 10.1021/bc900008s

Figure 6. Kinetics of triplex adduct formation.

Figure 6

(a) Equal volumes of solutions containing 1 μM TFO 1d and DNA duplex 4RY in triplex buffer were mixed and the absorbance at 260 nm was recorded as a function of time at 37°C. (b) Solutions containing 1 μM 32P-labeled duplex 4RY and 2 μM t2d were incubated in triplex buffer at 37°C for various lengths of time and analyzed by PAGE on a 20% denaturing gel. The percent adduct formation was determined as a function of time.