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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Bioconjug Chem. 2009 Dec;20(12):2222–2230. doi: 10.1021/bc900008s

Figure 7. Effect of pH on adduct formation by platinated deoxyribo- and 2′-O-methylribo-TFOs.

Figure 7

(a) Solutions containing 1 μM duplex 4RY and 2 μM platinated deoxyribo-TFOs t1d, t2d, or t3d in pH 6.5 or pH 7.4 triplex buffer were incubated at 37°C for 48 hrs and then subjected to 20% denaturing PAGE. (b) Solutions containing 1 μM duplex 4RY and 2 μM platinated 2′-O-methylribo-TFOs t2mr or t3mr in pH 6.5 or 7.4 triplex buffer at 37°C for 48 hrs and then subjected to 20% denaturing PAGE. The percent adduct formation as determined by phosphorimaging is shown below each lane. The purine strand of duplex 4RY is labeled in each case.