Figure 4. GluRs preferentially clusters opposite Brp-positive active zones in the rab3rup mutant when DGluRIII is limiting.
(A) Image of a rab3rup NMJ formed on a muscle in which GluRs were reduced by expression of a transgenic RNAi construct against DGluRIII driven by 24B-Gal4. NMJs were co-stained with antibodies against Brp (red) and DGluRIII (green). Scale bar represents 2 μm.
(B) Histogram shows quantification of the average area of Brp-positive and Brp-negative DGluRIII clusters in the rab3rup mutant under reduced DGluRIII conditions. n = 10; *p≪0.001.
