Figure 2. Phospho-S6 levels in RGCs of SOCS3^f/f mice with AAV-GFP or AAV-Cre after optic nerve injury.

(A, B) Immunofluorescence analysis with anti-p-S6 or TUJ-1 antibodies of the retinal sections from SOCS3^f/f mice injected with AAV-GFP (A) or AAV-Cre (B) at different time points post-crush. Scale bar, 50 µm.

(C) Quantification of p-S6⁺ RGCs. Data is presented as mean percentages of p-S6⁺and TUJ1⁺ cells among total TUJ1⁺ cells in the ganglion cell layer of each retina. Cell counts were performed on at least 4 non-consecutive sections for each animal, from four or five mice per group. *: p<0.01 by Dunnett’s test.