(A–D) Confocal images of optic nerves showing CTB-labeled axons around the lesion ites at 14 days post crush injury (dpc) from SOCS3f/f mice with AAV-GFP (A, B) or AV-Cre (C, D) and subsequent injection of PBS (A, C) or CNTF (B, D). *: crush site. Scale bar: 100 µm.
(E, F) Quantification of regenerating axons at different distances (250–1000 µm in E, and 1500–2500 µm in F) distal to the lesion sites at 14 days after crush injury. At least 5 different sections (every 4th section) from each animal were quantified. There were significant differences between the SOCS3f/f with CNTF group and the other three groups (ANOVA with Bonferroni’s post-test, p < 0.05 for each distance, 3 animals in each group).