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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Hear Res. 2009 Jan 11;249(1-2):15–22. doi: 10.1016/j.heares.2008.12.008

Figure 3. Western blot analysis and quantification graph of Notch1, NICD, Hes1, and Hes5 in normal and drug-damaged organ of Corti.

Figure 3

Notch1, NICD, Hes1, and Hes5 all appeared to rise after a lesion, with the first three peaking at 2 weeks and Hes5 appearing to peak at one month following ototoxicity. The Notch1 blot exhibited bands at ~280 kDa corresponding to the whole receptor, ~120 kDa corresponding to the cleaved portion (NICD), and one faint non-specific band at ~250kDa. The Hes1 blot exhibited the predicted band at ~30 kDa and no nonspecific bands. The blot to Hes5 exhibited the predicted band at ~20 kDa and one faint nonspecific band at ~40 kDa. GAPDH was used as a loading control for relative quantification of protein amounts with ImageJ (graph at right). N = normal, 1d = 1 day following the lesion, 14d = 14 days after the lesion, 30d = thirty days after the lesion, 60d = 60 days after the lesion.