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. Author manuscript; available in PMC: 2010 Dec 8.
Published in final edited form as: Mol Cancer Res. 2009 Dec 8;7(12):1928–1936. doi: 10.1158/1541-7786.MCR-09-0372

Figure 5. Curcumin inhibits ectopic fVII production at the protein level.

Figure 5

(A) fVII secreted in cell-culture supernatant from hepatic cancer cells cultured with or without curcumin was measured by a chromogenic activity assay based on fXa generation activity. Columns, mean; Bars, SD (n = 3). (B) Unlike hepatic cell, ectopically produced fVII is not secreted in cell-culture media (upper). Conditioned media for each cell-type (2 × 106 cells) were prepared for immunoblotting. Detection of ectopically expressed fVII in whole cell lysate of YMB-1 cells and its inhibition by curcumin (lower). (C) A procoagulant activity on YMB-1 cells cultured in the presence or absence of curcumin was measured by fXa generation assay. Columns, mean; Bars, SD (n = 3). **P < 0.005 different from secreted fVII level from HUH cells (*).