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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Immunother. 2009 Oct;32(8):826–836. doi: 10.1097/CJI.0b013e3181ad762b

FIGURE 4.

FIGURE 4

Expression and selection of 2 transgenes from 2 plasmids. PBMCs were cotransfected with pIRII-eGFP (5 μg), pIRII-ΔCD19 (5 μg), and pCMV-PB (5 μg). The co-transfected cells were stimulated with CD3/28 MAbs in the presence of IL-15. A, The cells were analyzed for GFP and CD19 expression by flow cytometry on day 1 and weekly thereafter. B, On day 8 a fraction of the T cells were selected for expression of CD19 using magnetic beads, and then immediately stimulated on CD3/28 MAbs-coated plates in the presence of IL-15 for 2 weeks. The cells were analyzed for GFP and CD19 expression on day 22. At this time the T-cell phenotype of selected and unselected cells were compared using the subset markers, CD4 and CD8, and memory markers, CD28 and CD62L, as shown in C and D.