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Down-regulation of Bmi1 and its effects on its target gene expression in human HIC cells. (A) Real-time RT-PCR analysis of Bmi1, p14Arf, and p16Ink4A in pLKO.1 or Bmi1/pLKO.1 infected HCC cells; (B) Protein expression of Bmi1 and p16 in pLKO.1 or Bmi1/pLKO.1 infected HCC cells. Actin was used as loading control.
