Figure Three.

Silencing Bmi1 expression leads to decreased cell growth in human HCC cells. (A) Cell growth assays in three HCC cell lines infected with lentivirus encoding pLKO.1 or Bmi1/pLKO.1. Cell numbers were counted at 3 days post infection; (B) Down-regulation of cell cycle genes: Mad2, Cdc2, Cdc20, and Bub1 in Bmi1/pLKO.1 infected Huh7 cells; (C) BrdU labeling assay showing a decrease of proliferation in Bmi1/pLKO.1 infected Hep3B cells; (D) Representative images of cell cycle distribution of Hep3B cells infected with pLKO.1 or Bmi1/pLKO.1 measured by PI staining and FACS analysis. The percentage of cells in each cell cycle phase is listed on the right panel. **: p <0.01; *: p <0.05.