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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Mol Cancer Res. 2009 Nov 24;7(12):1937–1945. doi: 10.1158/1541-7786.MCR-09-0333

Figure Seven.

Figure Seven

Regulation of p16Ink4A and p19Arf expression by RasV12 and/or Bmi1 in primary mouse hepatocytes. (A) Primary mouse hepatocytes were infected with adenovirus encoding EGFP or RasV12-HA. Western blotting shows the expression of HA-tagged RasV12 (left), and real-time RT-PCR shows the quantification of p16Ink4A and p19Arf expression in adenoviral infected primary hepatocytes (right); (B) Primary mouse hepatocytes were transfected with plasmids encoding EGFP, RasV12-HA and/or Bmi1-V5. Western blotting shows the expression of HA-tagged RasV12, V5-tagged Bmi1 and p16Ink4A (left). Arrow shows the ectopically expressed Bmi1-V5 that migrates higher than endogenous Bmi1 on gel. IMR90 cell lysate was used as a positive control for p16Ink4A protein expression. Quantification of p16Ink4A and p19Arf mRNA expression in transfected primary hepatocytes was performed using real-time RT-PCR (right). **: p <0.01; *: p <0.05.