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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Immunother. 2009 Oct;32(8):803–816. doi: 10.1097/CJI.0b013e3181ad4092

FIGURE 2.

FIGURE 2

Effects of RNAi in OVCAR3 cells. A, Flow cytometry analysis was performed to determine the inhibition of IFNAR1 or 2 expression levels and dose dependence 48 h after RNAi transfection. The expression ratios (%) were obtained by subtracting the ratios of the negative control (mouse IgG1 or mouse IgG2a). The data are the average ± standard deviation (n = 2). (upper, effects of IFNAR1/2-RNAi; lower, specificity of IFNAR1/2-RNAi) B, Western blot analysis was performed 48 h after transfection of RNAi. The relative expression ratios (%) were determined by quantification of the western blot data followed by comparison to the values of the respective β-actin. RNAi indicates RNA interference; IFNAR, interferon (alpha, beta and omega) receptor; IgG, immunoglobulin G; Jak1, Janus kinase 1; Tyk2, tyrosine kinase 2; IRF9, interferon regulatory factor 9; Stat, signal transducers and activators of transcription.