Figure 3.

(A) Growth of R2-PIM8(smt) cells containing pLACPB2 (filled symbols) or pZIA (open symbols) in Allens medium supplemented with 0 (circles), 2 (squares), or 10 (triangles) μM Zn²⁺. Cells were inoculated at a density of 1 × 10⁶ cells ml⁻¹, and growth was monitored by measuring the OD₅₄₀. Data points represent the mean values from three separate cultures with SD (values too small to be visible above or below symbols). (B) ⁶⁵Zn accumulated by R2-PIM8(smt) cells containing pLACPB2 (first bar in each pair) or pZIA (second bar in each pair) exposed to 2, 5, or 10 μM Zn²⁺ for 1 h. Data points represent the mean values from three separate cultures with SD. (C) Colonies of wild-type Synechocystis PCC 6803 (top left), the ziaA mutant (bottom center), or ziaA-restored cells (top right) were streaked onto Kratz and Myers medium supplemented with 0 or 14 μM Zn²⁺.