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. 1998 Sep 1;95(18):10728–10733. doi: 10.1073/pnas.95.18.10728

Figure 5.

Figure 5

(A) β-Galactosidase activity in R2-PIM8(smt) cells containing pLACPB2 (P−), pLAC549 (ZiaR), pLAC549-AMB (ZiaR−), pLAC886 (ZiaR/sll0793), or pLAC886-AMB (ZiaR−/sll0793). Cells were grown with no metal supplement (first bar in each pair) or in the presence of Zn2+ (2 μM) for ≈20 h immediately before assay (second bar in each pair). The data points represent the means of three separate assays with SD. (B) Gel retardation assays were performed by using 3 fmol of 160-bp zia operator–promoter as probe with ≈36 μg of protein extract from cells used in A. The levels of poly(dI-dC)⋅poly(dI-dC) used and the position of free probe (FP) are indicated.