Figure 9.

Overexpression of Myc-Lin7c leads to the formation of cellular rosettes in the neural tissue. Myc-Lin7c mRNA was injected at the one- to four-cell stages. Embryos were fixed at ∼12-ss. Arrows indicate the apical regions of cellular rosettes. These regions are enriched with ZO-1 and Myc-Lin7c. The inset shows a magnification of a rosette. At this stage, most of the expressed Myc-Lin7c localized diffusely in the cells, presumably because the apical targeting mechanism is not fully in place. Nevertheless, apical accumulation of Myc-Lin7c is already detectable. Scale bars, 30 μm.