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. Author manuscript; available in PMC: 2010 Jan 5.
Published in final edited form as: Leukemia. 2009 May 7;23(9):1667–1678. doi: 10.1038/leu.2009.91

Figure 3. STAT3 localization in primary biopsy and in MEC04 cells.

Figure 3

(A) Immunochemistry with anti-Y705-STAT3 shows a constitutive phosphorylation of Y705 STAT3 with a nuclear localization in primary cells from a gastric biopsy (brown staining) (original magnification, × 200, Zeiss Axioscope). (B) Evidence of STAT3 translocation in MEC04 cell line by confocal microscopic analysis. MEC04 cells were washed and were resuspended in culture milieu without rIL-2 overnight. Cells were fixed, permeabilized, and stained with DAPI (nucleus marker, blue), with anti-STAT3 (1:200), and visualized with anti-mouse PE (red)-labeled secondary antibody (1:100). Images were acquired on a Leica TCS SP2 laser scanning confocal microscope (Leica Microsystems, Heidelberg GmbH) equipped with a HCX PL Apochromat CS 63×1.40 Oil objective, using Leica Confocal Software (version 2.61). STAT3 is mainly detected in nucleus in MEC04 cells only. In contrast, control NK cells exhibit a cytoplasmic pattern of STAT3 staining.