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Morphological analysis of the kinetics of extracellular differentiation from tissue-derived trypomastigotes (a) to amastigotes (h), in hgDMEM at pH 5 without FBS at 37°C: intermediate forms at 1 hour (b), 2 hours (c), 3 hours (d), 4 hours (e), 5 hours (f), and 6 hours (g) of transformation stained with Hema 3. Bar = 25 μm.
